ないのよ

there isn't

→ 無い
phrase Common #4,056
there isn't Adds an explanatory or emphatic tone to ない. The ending のよ is often associated with feminine speech, though it is not exclusively used by women.
JA: 今日は時間がないのよ。
EN: I don't have time today.

あなたは許す立場にないのよ

You’re in no position to forgive anyone.

— どん底作家の人生に幸あれ! (2019)

トムは私なんか眼中にないのよ。

Tom doesn't even notice me.

今、お金ないのよ。

I don't have any money right now.

私、門限ないのよ。

I don't have a curfew.

彼女の方はその気がないのよ

She’s not interested.

— 動くな、死ね、甦れ! (1990)

女の子達は見る目がないのよ

Girls just don’t know how to judge people.

— キャッチ・ミー・イフ・ユー・キャン (2002)

両方はないのよ

You can't have both.

— Pompo the Cinephile (2021)

時間ないのよ

I don't have time.

— One Cut of the Dead (2017)

今はもうないのよ

It’s not there anymore.

— M - Eine Stadt sucht einen Mörder (1931)